Project Overview

lauber_88_soils

   metagenomes   project metadata

VisibilityPublic
Static Linkhttp://metagenomics.anl.gov/linkin.cgi?project=68

Description

Soils harbor enormously diverse bacterial populations, and soil bacterial communities can vary greatly in composition across space. However, our understanding of the specific changes in soil bacterial community structure that occur across larger spatial scales is limited because most previous work has focused on either surveying a relatively small number of soils in detail or analyzing a larger number of soils with techniques that provide little detail about the phylogenetic structure of the bacterial communities. Here we used a bar-coded pyrosequencing technique to characterize bacterial communities in 88 soils from across North and South America, obtaining an average of 1,501 sequences per soil. We found that overall bacterial community composition, as measured by pairwise UniFrac distances, was significantly correlated with differences in soil pH (r = 0.79), largely driven by changes in the relative abundances of Acidobacteria, Actinobacteria, and Bacteroidetes across the range of soil pHs. In addition, soil pH explains a significant portion of the variability associated with observed changes in the phylogenetic structure within each dominant lineage. The overall phylogenetic diversity of the bacterial communities was also correlated with soil pH (R2 = 0.50), with peak diversity in soils with near-neutral pHs. Together, these results suggest that the structure of soil bacterial communities is predictable, to some degree, across larger spatial scales, and the effect of soil pH on bacterial community composition is evident at even relatively coarse levels of taxonomic resolution.

Funding Source

National Science Foundation (MCB0610970 and DBI0301773) and the Andrew W. Mellon Foundation.

Contact

Administrative
Christian Lauber (Christian.lauber@colorado.edu)
Cooperative Institute for Research in Environmental Sciences, University of Colorado (http://cires.colorado.edu)
Boulder, CO, USA

Technical
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- (http:// - )
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Additional Data

center_project_namePyrosequencing_based_assessment_of_soil_pH_as_a_predictor_of_soil_bacterial_community_structure_at_the_continental_scale
study_typemetagenome
study_titlePyrosequencing_based_assessment_of_soil_pH_as_a_predictor_of_soil_bacterial_community_structure_at_the_continental_scale
sff_completey
qiime_idqiime:103
metadata_completey
study_descriptiontargeted gene survey
investigation_typebacteria_archaea
miens_complianty
center_nameCCME
study_aliasLauber88Soils
sample_count89
pmid19502440

Metagenomes